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REAGENTS & PROTOCOLS

REAGENTS

  • Cellmatrix Type I-A (Wako Chemicals, #637-00653) VIEW

  • Rat Collagen I (LV) Lower Viscosity (Trevigen - Cultrex, #3443-100-01) VIEW

PLEASE NOTE: because of the volume of requests for the stable expressing Rspo1-Fc cell line, distribution has now been turned over to Millipore, a Sigma-Aldrich Brand. For purchase, order online (Catalog #SCC111).

 

Requests for reagents or mice should be sent to Calvin at cjkuo@stanford.edu. We typically require a simple MTA.

PROTOCOLS

  • Adenovirus production protocol DOWNLOAD PDF

  • Culture of 293T cells stably expressing RSpo1-Fc DOWNLOAD PDF

  • Production of R-Spondin 1-conditioned medium - a Millipore Protocol VIEW

SOME REVIEW ARTICLES

  • Regulation of the Blood-Brain Barrier in Health and Disease. Cara C. Rada, Kanako  Yuki, Jie Ding, Calvin J. Kuo. Cold Spring Harb Perspect Med. 2023 Mar 27:a041191. doi: 10.1101/cshperspect.a041191. PMID: 36987582. LINK

  • Immune organoids: from tumor modeling to precision oncology. Vinh Dao, Kanako Yuki, Yuan-Hung Lo, Michitaka Nakano, Calvin J. Kuo. Trends In Cancer. 2022. 8 (10): 870-880. doi: 10.1016/j.trecan.2022.06.001. LINK

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