REAGENTS & PROTOCOLS
REAGENTS
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Cellmatrix Type I-A (Wako Chemicals, #637-00653) VIEW
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Rat Collagen I (LV) Lower Viscosity (Trevigen - Cultrex, #3443-100-01) VIEW
PLEASE NOTE: because of the volume of requests for the stable expressing Rspo1-Fc cell line, distribution has now been turned over to Millipore, a Sigma-Aldrich Brand. For purchase, order online (Catalog #SCC111).
Requests for reagents or mice should be sent to Calvin at cjkuo@stanford.edu. We typically require a simple MTA.
PROTOCOLS
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Culture of 293T cells stably expressing RSpo1-Fc DOWNLOAD PDF
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Production of R-Spondin 1-conditioned medium - a Millipore Protocol VIEW
SOME REVIEW ARTICLES
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Regulation of the Blood-Brain Barrier in Health and Disease. Cara C. Rada, Kanako Yuki, Jie Ding, Calvin J. Kuo. Cold Spring Harb Perspect Med. 2023 Mar 27:a041191. doi: 10.1101/cshperspect.a041191. PMID: 36987582. LINK
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Immune organoids: from tumor modeling to precision oncology. Vinh Dao, Kanako Yuki, Yuan-Hung Lo, Michitaka Nakano, Calvin J. Kuo. Trends In Cancer. 2022. 8 (10): 870-880. doi: 10.1016/j.trecan.2022.06.001. LINK
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An expanded universe of cancer targets. William C Hahn, Joel S Bader, Theodore P Braun, Andrea Califano, Paul A Clemons, Brian J Druker, Andrew J Ewald, Haian Fu, Subhashini Jagu, Christopher J Kemp, William Kim, Calvin J Kuo, Michael McManus, Gordon B Mills, Xiulei Mo, Nidhi Sahni, Stuart L Schreiber, Jessica A Talamas, Pablo Tamayo, Jeffrey W Tyner, Bridget K Wagner, William A Weiss, Daniela S Gerhard. Cancer Target Discovery and Development Network. Cell 2021 Mar 4;184(5):1142-1155. doi: 10.1016/j.cell.2021.02.020. PMID: 33667368